Contact an Account ManagerSensitive and selective determination of picogram amounts of ciprofloxacin and its metabolites in biological samples using HPLC and photothermal postcolumn derivatization
Summary
Type: HPLC
Application #: 103180
Catalog#: 720041.40 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4x250mm
Detection: fluorescence, excitation 278nm, emission 445nm
Conditions: eluent: 4% aqueous tetrabutylammonium bisulphate - acetonitrile (7 - 10% CH3CN for ciprofloxacin and M1, 25 - 30% CH3CN for M2, M3 and M4)
flow rate 2ml/min
Authors: Scholl H
Source: J Chromatogr 416 (1987) 321 - 330
Details
Legend: Fig. 3. Chromatograms of ciprofloxacin and metabolites with postcolumn
derivatization: injection of a) standard solutions, b) serum after dilution by
a factor of 3.33 (top) or 5 (bottom) and (c) uri